DISEASES DIVERSITY FOR FLAX GENETIC RESOURCES IN LATVIA

Inga Stafecka, Dace Grauda, Veneranda Stramkale

Abstract


Flax (Linum usitatissimum L) yield of stem and seeds and them quality is influenced by a number of harmful diseases but investigation about pathogens in recent years have not been done in Latvia. Each stage of development of disease is important in the pathogen life cycle and requires certain condition. Goal of this study have identify possibilities of the pathogens and were assess disease severity depending on the genotypes on flax in variable environmental conditions. The resistance to the diseases for 24 flax genotypes and standard variety ‘Vega 2’ were evaluated. The field trials have been carried out over the period from 2015 to 2016 at the Research Centre of Priekuli, part of Vilani in Latgale. Disease progress was measured every week, and for each treatment, severity of diseases index and the area under the disease progressive curve (AUDPC) were calculated. The flow cytometry method for detection of flax pathogens was developed. The Fusarium avenaveum was dominating disease and statically significant in both vegetation periods. In 2015 were identified statically significant (p<0.001) highest AUDPC the casual agents powdery mildew (Oidium lini) and of stem break and browning caused by Polyspora lini but in 2016 anthracnose (Colletotrichum lini), pasmo (Septoria linicola) and fusarium wilt caused by fungus Fusarium oxysporum f. sp. lini. The resistance of Colletotrichum lini, Fusarium oxysporum f. sp. lini, Septoria linicola and Oidium lini have flax variety of ‘Rezekne’ in both years.

Keywords


AUDPC; diseases; flax; flow cytometry; pathogens

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References


X. Beaudoin, Disease and pest control. In: G. Marshall (Ed.): Flax: Breeding and Utilisation. Advances in Agricultural Biotechnology 25, Kluwer Academic Publishers, the Netherlands, pp. 81-88. 1989.

L. C. Sharma and R. L. Mathur, Variability in first single spore isolates of Fusarium oxysporum f.sp. lini in Rajastan. Indian Phytopathology 24:688-704, 1971.

K. Y. Rashid, Principal diseases of flax. In: Muir, A.D., Westcott, N.D. (Eds.), Flax: The Genus Linum. Taylor and Francis Ltd, New York, NY, 2003 pp. 92–123.

R. M. Kozlowski, Hanbook of natural fibres. Woodhead Publishing Series in Textiles: Number 118, Volume 1, 2012, pp 390 – 523.

H. Booker, R. Bueckert, S. Duguid, J. Gavloski, R. Gulden, R. Dueck, … A. Ulrich, „Growing Flax Guide”, 2014 Retrieved January 20, 2017from http://flaxcouncil.ca/growing-flax/growth-and development/

K. Heller, Crop management of fibre flax in Europe, Presentation Summer school, Catania – Italy, 2013. Retrieved January 18, 2017 http://www.fibrafp7.net/Portals/0/06_Heller.pdf.

L.M. Hall, H. Booker, R. M. P. Siloto, A. J. Jhala, R. J. Weselake, Flax (Linum usitatissimum L.) Industrial Oil Crops, First Edition, 2016, pp 157-194.

K. Y. Rashid and E. O. Kenaschuk, Effect of trifluralin on fusarium wilt in flax. Can. J. Plant Sci. 1993, 73: 893-901.

I. Simko and H. P. Piepho, The Area Under the Disease Progress Stairs: Calculation, Advantage, and Application Phytopathology Vol. 102, No.4, 2012 pp 381-389.

V. Haridasa, S. Ranjbara, I. A. Vorobjevc, A. E. Goldfelda, N. S. Bartenevaa, Imaging flow cytometry analysis of intracellular pathogens. Methods. Volume 112, 1 January 2017, Pages 91–104 Flow Cytometry.

L.G. Chitarra; van den Bulk, R.W. The application of flow cytometry and fluorescent probe technology for detection and assessment of viability of plant pathogenic bacteria. Eur. J. Plant Pathol. 2003, 109, 407–417.

Н. И. Лошакова, Т. В. Крылова, Л. П. Кудрявцева, „Методические указания по фитопатологической оценке устойчивости льна-долгунца к болезням” – Москва,– 52 с. 2000.

J. E. Van der Plank, „Plant Diseases: Epidemics and Control.” Academic Press, New York. 1963.

I. Arhipova, un S. Bāliņa, „Statistika ekonomikā. Risinājumi ar SPSS un Microsoft Excel.” (Statistics in the economy. The solutions with SPSS and Microsoft Excel.) 2. iss. Rīga: Datorzinību Centrs, 2006, pp. 364. (in Latvian).

M. C. Leach, S. L. A. Hobbs, „Plantwise knowledge bank: delivering plant health information to developing country users.” Learned Publ 26: 180–185, 2013.

E. Lysøe, L. J. Harris S. Walkowiak R. Subramaniam H. H. Divon E. S. Riiser C. Llorens T. Gabaldón H. C. Kistler W. Jonkers A. K. Kolseth K. F. Nielsen U. Thrane R. J. Frandsen „The genome of the generalist plant pathogen Fusarium avenaceum is enriched with genes involved in redox, signaling and secondary metabolism.” PLoS One. 2014 Nov 19;9(11).

K. Bačelis, E. Gruzdevienė, Resistance of fibre flax varieties to Colletotrichum lini Manns et Bolley // Biologija. – 2001. – Nr. 3, p. 4–7. (ISSN: 1392-0146) (http://www.elibrary.lt/resursai/LMA/Biologija/B-033.pdf).

E. Gruzdevienė, The Peculiarities Of The Occurrence Of Pasmo Disease (Septoria linicola) In Central Lithuania Zemdirbyste-Agriculture, vol. 95, No. 3 (2008), p. 58–64.

K. Y. Rashid, K. L. Bailey, B. D. Gossen, R. K. Gugel, and R. A. A. Morrall, Diseases of flax. Diseases of Field Crops in Canada, 3rd ed. eds. University Extension Press, Saskatoon, SK, Canada, 2003. Pages 147-154

W. E. Saskston, „Pasmo – past, present, and future.” In: Proceedings of the Flax Institute of the United States, November 19th, 1959, Fargo, North Dakota, U.S.A., 1959 pp. 3–5.

R. E. Beale, „Studies of resistance in linseed cultivars to Oidium lini and Botrytis cinerea.” Aspects Appl. Biol. 1991, 28:85–90.

G. S. Saharan and M. S. Saharan, Studies on powdery mildew of linseed caused by Leveillula taurica (Lev.). Indian J. Mycol. Plant Pathol. 1994, 24:107–110.

K. Rashid and S. Duguid., Inheritance of resistance to powdery mildew in flax. Can. J. Plant Pathol. 2005, 27:404–409.

E. Gruzdeviene, General problems and control of fungal diseases of flax in Lithuania // Development of Environmentally Friendly Plant Protection in the Baltic Region: proceedings of the International Scientific Conference. – Tartu, 2000, p. 40-42.

R. T. Gregory, J. A. Nicol, H. Tamm, B. Kullman, K. Kullman, I. J. Leitch, B. G. Murray, D. F. Kapraun, J. Greilhuber and M. Bennet, „Eukaryotic genome size databases”. Nucleic Acids Res. 35, (2007) 332– 338.

L. D’Hondt, M. Höfte V. E. Bockstaele and L. Leus Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status. Molecular Plant Pathology. 2011 pp 1-14.




DOI: http://dx.doi.org/10.17770/etr2017vol1.2548

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