EVAIUATION OF THE GENOTOXIC EFFECTS OF SOME BIOLOGICALY ACTIVE SUBSTANCES

Jēkabs Raipulis, Malda Maija Toma

Abstract


There are more and more substances coming in contact with people and environment, they have got genotoxic features, which cause hereditary illnesses and tumors. Though cancer treatment becomes more and more effective, still the amount of deaths from tumors caused by harmful substances are increasing from year to year. To reduce the effects of these harmful substances, they should be detected and avoided.

To perform experiments using Saccaromyces cerevisiae mutagenicity testsystem and Escerichia coli SOS chromotest to find out biologically active substances used in labs and industry, also its mixture or separate compounds possessing genotoxicity with other substances. The genotoxic properties of 11 substances were studied. Results showed that formaldehyde, urethane, acridine orange, acridine yellow, furazolidone, nalidixic acid, hydrogen peroxide, potassium bichromate, was a strong inducers of the genotoxic disorders.


Keywords


genotoxins; genotoxicity; mutagens; carcinogens; E.coli SOS chromotest

Full Text:

PDF

References


Douglas, M.P., Rogers, S.O. DNA damages caused by common cytological fixatives. Mut. Res. 1998, V 401, pp. 77-88.

Ptitsyn, L.R., Horneck, G., Komova, O., Kozubek, S., Krasavin, E., Boney, M., Rettberg, P. A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells. Appl. Env. Microbiology, 1997. V 63, pp. 437-4384.

La Curieux, F., Marzin, D., Erb, F. Comparision of three short-term assays: results on seven chemicals. Potential contribution to the water genotoxicity. Mut research, 1993. V 319, pp. 223-236.

Speit, G., Merk, O. Evaluation of mutagenic effects of formaldehyde in vitro: detection of crosslinks and mutations in mouse lymphoma cells. Mutagenesis , 2002. V. 17, p. 183-187.

Toma, S., Raipulis, J. The recombinogenic and mutagenic influence of formaldehyde on yeast Saccaromyces cerevisiae. Proc. Latv. Acad, Sci, 1996. V 50, p. 126-129.

Marrin, D., Olivier, P., Vorhi, H. Kinetic determination of enzymatic activity and modification of metabolic activation system in the SOS chromotest. Mutation Res., 1986. V. 164, p. 353-359

Quillander, P., Hofnung, M. The SOS chromotest: areview. Mutation Research, 1993. V 297, pp. 235-248.

Schmidt, E., Goegelmann, W., Bauchinger, M. Formaldehyde induced cytotoxic, genotoxic and mutagenic response in human lymphocytes and Salmonella typhimurium. Mutagenesis, 1986, V. 1 p. 427-431.

Grafstrom, R.C., Hsu, C.J., Harris, C.C. Mutagenicity of formaldehyde in Chinise hamster lung fibroblasts: sinergy with ionizing radiation and N-nitroso-N-methylurea. Chem.-Biol. Interaction, 1993. V. 86.

McLaughlin, J.K.. Formaldehyde and cancer: a critical review. Int. Arch. Occup. Environ. Helth., 1994. V. 66, p. 295-301.

Svensson, K. Studies of to vinyl chloride, urethane, 1,2-dichlorourethane and propone with respect to metabolism, covalent binding to macromolecules and genotoxic risk. Univ. Stocholm, 1988. 69 p.

Auro, A., Sumano, H., Ocampo, L., Barragan, A. Evaluation of the carcinogenic effects of furazolidone and its metabolites in two fish species. Pharmacogenomics, 2004. V 4, pp. 24 -28.

Guzella, L., Monarea, S. Zani, C., Feretti, D. Zerbini I. et al. In vitro potential genotoxic effect of surface drinking water treated with chlorine and alternative disinfectants. Mut Res., 2004. V 564, pp. 179-193.

McDonnell, G., Russell, A. Antiseptics and disinfectants: activity, action and resistance. Clin Microbial. Review, 1999. V 12, pp. 147-197.

Pohl, L.R., Krishna, G. Deuterium isotope effect in bioactivation and hepatotoxicity of chloroform. Life Science, 1978. V 23, pp. 1067-1072.

Marczewska, J., Koziorowska, J. Effects of irone and cupper ions on the inducibility of hydrogen peroxide in E.coli. Acta Pol. Pharm, 2000. V 57, pp. 49-52.

Trosko, J., Uphom, B.L. The emperor wears no clothes in the field of carcinogen risk assessment: ignored concepts in cancer risk assessment. Mutagenesis, 2005. V 20, pp. 81-92.




DOI: https://doi.org/10.17770/etr2007vol1.1738

Refbacks

  • There are currently no refbacks.


SCImago Journal & Country Rank